Exosome

Extracellular Vesicle isolation and Storage TEchnology Platform
EViSTEP^™

Good-bye Manual Liquid Handling,
Hello Automated Exosome/Extracellular Vesicles Isolation

Our fully automated technology provides solutions
for isolation of exosomes/extracellular vesicles isolation from a variety of clinical samples
with high purity and reproducibility.

Exosomes/Extracellular Vesicles (EVs) are attracting much attention as they are used in a wide range of medical and healthcare fields, including diagnostic and therapeutic areas.
EViSTEP ^™ (Extracellular Vesicle Isolation and Storage Technology Platform), which is based on our core technologies of pre-treatment reagent, high quality antibodies, and a fully automated EV isolating device, has achieved stable and highly efficient isolation of EVs from a variety of samples.
Our EViSTEP^™ instrument provides solutions for the development of your Exosome research.
Feel free to contact us for more information.

What are Exosmes ?

The Exosome

Exosomes, a type of extracellular vesicles (EVs), are secreted into extracellular fluids and are found in most body fluids, including blood, urine, saliva, breast milk, and cerebrospinal fluid. A number of biologically active molecules including proteins, mRNA, miRNA, DNA and phospholipids are found in exosomes. Exosomes are thought to play key roles in cell-to-cell communication and have been associated with various disease conditions, e.g., cancer metastasis and vascularization. Thus exosome studies have great potential for the development of applications in diagnostics and novel therapies.

Applications of exosome research

About Autoevis^™

Automated EV Isolating Device with our unique reagent and antibody Autoevis™

Specifications

Principle	EV isolation using Abs-conjugated beads
Sample size	up to 500 μL (serum, plasma, urine saliva, culture medium, etc.)
Number of samples	Maximum 6 / run
Run time	60 minutes

The Autoevis^™ workflow

❶Add our unique reagents to the samples, then the samples are added to antibody-conjugated magnetic beads
❷Immunoprecipitate EVs from samples
❸Magnetize and collect the beads to remove the supernatant
❹Wash the beads
❺-1Elute EVs or
❺-2Lyse EVs and collect the contents

Advantages of EViSTEP^™

Below are our experimental data for EViSTEP^™

More EVs isolated with EViSTEP^™

Far Higher Expression Level of EVs-derived β-actin mRNA

Much Higher Expression Level of EVs-derived β-actin mRNA

Higher Isolation Efficiency with EViSTEP^™ from Various Clinical Samples

Significant Increase in Detection of RNA-based Biomarker Candidates in Serum

Comparison with Other Conventional Methods

	Ultracentrifugation	Affinity Interaction	Polymer-based Precipitation	Gel Filtration	EViSTEP^™
Reproducibility	-	++	+	++	++ ~ +++
Purity	-	++	-	++	++ ~ +++
Sample variety	++	- ~ +	++	++	++ ~ +++
Storage					++
High throughput/automation	-	+	+	+	+++

More Stable EV Storage with EViSTEP^™

EViSTEP^™ × H.U.Group Technology

EViSTEP^™ combined with our other technologies provides supports to expand your exosome studies.

※For Research Use Only.

Examples of business alliance and partnerships

1. Collaboration Discovery and Development

・EV isolation and analysis
・Biomarker search
・Liquid Biopsy/CoDx
・Functional EVs, Engineered EVs

2. Technology Transfer and Consulting

・Licensing and transfer of H.U.Group technology and intellectual property
・Data analysis services/Customized data analysis systems
・Call for research proposals

Collaboration

Collaborative Research with Baylor Genetics and Baylor College of Medicine

Researchers to study liquid biopsy test for cancer
https://www.bcm.edu/news/researchers-study-biopsy-test-cancer